The SISCAPA® Workflow
The SISCAPA workflow has been optimized to facilitate addition-only sample digestion and immuno-capture enrichment of parallel samples. The generic workflow can be used for a range of protein analytes in singleton- or multiplexed-format.
Implementation of this workflow on the Agilent Bravo Liquid Handling Platform provides high-precision processing of many hundreds of samples per day.
SISCAPA Workflow Tutorial
This step-by-step instructional video describes how the SISCAPA assay protocol has been implemented on a highly automated robotic platform for high precision and high throughput sample processing with minimal user intervention.
SISCAPA Workflow - Precision
The 96-tip head of the Agilent Bravo Liquid Handling Platform delivers an entire plate's worth of reagents in one step.
This highly parallel process provides unprecedented throughput for protein quantitation sample prep and also allows for precision in the order of single-digit coefficients of variation (CV).
Addition-only digestion and SISCAPA enrichment on Agilent Bravo
Digestion and SISCAPA steps do not add appreciably to overall % CV
SISCAPA Workflow - Throughput
The Agilent Bravo Liquid Handling Platform allows parallel processing of 96 samples at a time.
Significant enrichment of the target peptide analyte and removal of matrix facilitates abbreviated, or eliminated, LC separation of samples ahead of quantitation via mass spectrometry.
Several hundred samples can be prepared and quantitated in an eight hour work day.
SISCAPA Workflow - Simple User Interface
The Agilent Bravo Liquid Handling Platform utilizes a simple user interface that provides instructional interaction for the non-expert user.
The Workflow Navigator guides the user through each of the three steps of the SISCAPA workflow and the Method pages instruct the user how to set up the Bravo robotic deck with the plates, tips and reagents needed to execute the workflow in a highly automated manner.