SISCAPA = Stable Isotope Standards and Capture by Anti-Peptide Antibodies
SISCAPA technology is the smart shortcut to sensitive quantitation of protein biomarkers and targets. SISCAPA assays combine the precision of MRM mass spectrometry with the power of affinity enrichment to deliver a superior alternative to conventional immunoassays for protein quantitation. The SISCAPA workflow is highly automated and exploits familiar LC-MS/MS platforms widely used for drug and metabolite quantitation.
SISCAPA provides a range of practical advantages over conventional ligand binding immunoassays:
Sensitivity SISCAPA improves peptide multiple reaction monitoring (MRM) sensitivity by 3-4 orders of magnitude over non-enriched samples.
Specificity SISCAPA combines antibody immunocapture selectivity with the near-absolute structural specificity of MRM mass spectrometry.
Standardization SISCAPA employs true internal standards (stable isotope labeled synthetic peptides) within each assay for reliable quantitation.
Multiplexing SISCAPA assays can be combined in mix-and-match panels without cross-reactions common in sandwich immunoassays.
Throughput SISCAPA delivers highly purified peptide analytes, free of matrix components, for decreased LC times and higher throughput.
Development SISCAPA assay development is faster, less expensive and more straightforward than sandwich immunoassay development.
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