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A better handle on protein biomarkers


... or how to measure proteins accurately and efficiently in complex samples

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A better handle on protein biomarkers


... or how to measure proteins accurately and efficiently in complex samples

 

Why this works

SISCAPA technology combines the best features of mass spectrometry and immunoassays to provide a faster, better, cheaper way to measure amounts of specific target proteins in complex samples like serum and whole blood.  

SISCAPA assays provide a smooth path from biomarker validation to use in drug development and clinical applications, all using the same instruments and reagents.

 
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Detect the right molecule


Sequence-specific direct analyte detection

Detect the right molecule


Sequence-specific direct analyte detection

SISCAPA eliminates protein interactions that can cause false positives, false negatives and other interferences in conventional immunoassays by digesting sample proteins to peptides in the first step of the workflow. 

Selected proteotypic peptides representing the protein targets are then extracted and measured in relation to a stable isotope labeled internal standard using mass spectrometry, a method that verifies the chemical structure (sequence) of a peptide directly.

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Dive deeper


Access proteins of very low abundance using specific affinity enrichment of target peptides from sample digest.

Dive deeper


Access proteins of very low abundance using specific affinity enrichment of target peptides from sample digest.

The simple act of collecting all of a low abundance peptide from a large digest sample and presenting it to the mass spectrometer in purified form extends MS assay sensitivity by 3-4 orders of magnitude.

Proteins far below mass spec detection limits in an unfractionated digest can be measured.

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Mix and match panels


Combine assays without cross-assay interference, including very high and very low abundance proteins, in a single test panel.

Mix and match panels


Combine assays without cross-assay interference, including very high and very low abundance proteins, in a single test panel.

SISCAPA assays do not interact or compete with one another, unlike conventional immunoassays where assay cross-talk inhibits use of multiplex panels.

Most assays utilize an unvarying standard workflow, incorporating two analyte specific reagents (a labeled peptide internal standard and the SISCAPA anti-peptide antibody) .  Proteins differing in abundance by 10,000,000-fold can be measured together in one panel by stoichiometric flattening.

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Analyze thousands of samples


Efficiently analyze large sample sets to achieve robust statistics on biological variation.

Analyze thousands of samples


Efficiently analyze large sample sets to achieve robust statistics on biological variation.

Fully automated SISCAPA protocols are available on several liquid handling robots, including full walk-away methods capable of processing two 96-well plates of samples in 4 hours.

MS detection of SISCAPA-enriched peptides is rapid (short sample-to-sample cycles) because of the vastly reduced sample complexity.

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Get running quickly


Select assay from the catalog, or commission rapid development of novel SISCAPA assays.

Get running quickly


Select assay from the catalog, or commission rapid development of novel SISCAPA assays.

A growing menu of assays for clinically relevant protein biomarkers is available off the shelf.

Custom assays for additional targets are developed by SAT on a fee-for-service basis.
 

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Eliminate chromatography


Purified analytes allow direct MS measurement without LC

Eliminate chromatography


Purified analytes allow direct MS measurement without LC

Liquid chromatography is a major factor limiting adoption of mass spectrometry for routine protein analysis: development of LC methods, long cycle times per sample, replacement of consumables (e.g., columns) and fluidic failures all contribute to cost and downtime.

SISCAPA analyte-specific enrichment eliminates most or all of the need for chromatographic separation, in many cases allowing direct MS quantitation without LC (e.g., by using RapidFire-MRM or MALDI-TOF), with sample-to-sample cycle times as short as 7-20 sec.

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Move beyond biomarker discovery


to measure biology... and ultimately make a clinical difference.

Move beyond biomarker discovery


to measure biology... and ultimately make a clinical difference.

Please feel free to contact us with any questions or comments:

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